Multiple Expression Systems
CBET is a purpose-built bioprocessing center with upstream and downstream laboratories that are equipped with the latest industry-grade technologies, including single-use and traditional systems capable of batch, continuous, and perfusion processes.
Students at CBET will complete hands-on projects across the biomanufacturing continuum, from cell line development, expression system selection, to optimizing media, scaling up to bioreactor inoculation, process monitoring, to collection and downstream purification.
CBET has bench-scale technology for cell culture and microbial fermentation processes to produce antibodies, recombinant proteins, enzymes, and other biologics. The center is also equipped for cell therapy and gene therapy development projects.
- Cell Culture
- Microbial Fermentation
- Cell Therapy
- Gene Therapy
- Procell Analytics / Quality Control
Upstream LAB & EQUIPMENT
Cytiva IN Cell Analyzer 2000
A widefield high-content imaging system designed for speed and throughput.
Eppendorf DASbox Mini
A mini-bioreactor system equipped with 2×4 150-mL cell culture and microbial reusable culture vessels.
Eppendorf BioFlo 320
BioFlo 320 bioreactors are equipped with both cell culture and microbial 3-L glass vessels. (Qty. 2)
Pall iCELLis Bioreactor
An automated, single-use, fixed-bed bioreactor that provides excellent growth conditions for adherent cells.
Beckman Coulter Vi-CELL Blu Cell Analyzer
Cell viability analyzer providing rapid and accurate cell analysis essential in most cell-culture bioprocesses.
Cytiva WAVE 25 Rocker
Integrated temperature sensors and load cells, designed for handling and control of cell cultures up to 25L bags.
Bio-Rad ChemicDoc MP
System for imaging and analyzing gels and western blots.
Invitrogen EVOS M5000 (ThermoFisher)
Fluorescence microscope with a high-resolution CMOS camera and the ability to capture three fluorescence channels.
Xcellerex XDR-10 (Cytivia)
Single-use stirred-tank bioreactor system for use in process development and small-scale production.
Downstream Lab & Equipment
ThermoFisher Vanquish UHPLC
Iron free flow path for the analysis of biomolecules at conditions demanding eluent with hand-tightened joints.
Cytiva ÄKTA flux S
Designed for sample concentration and diafiltration (buffer exchange) as well as cell harvest and clarification. (Qty. 2)
Cytiva ÄKTA Pure 25 M
Protein purification units with microgram-scale protein purifications in Tricorn columns up to milligram-scale.
Beckman Coulter Optima XPN 100
Ultracentrifuge equipped with titanium rotors that can provide centrifugation speeds up to 100,000 rpm.
Bio Tek Synergy H1
Configurable multi-mode microplate reader with both monochromator-based optics and filter-based optics.
Microvolume UV-Vis Spectrophotometer to quantify and qualify DNA, RNA, and protein samples with only 1-2 µL.
Bio-Rad CFX96 Touch Real-Time PCR
Six-channel (five colors and one FRET channel) real-time PCR instrument combines advanced optical technology.
- Western Blot Systems
- Protein Blot Modules
- DNA Gel Electrophoresis Systems
- Inverted Microscopes with Cameras
- Biosafety Cabinets
- Mammalian Cell Culture Incubators
- Shaking Bacterial Incubator
- Microbiological Incubator
- Refrigerated Centrifuges
- Ultralow -80 Freezer
- Liquid Nitrogen Dewar
- Bead Mill Homogenizer
- Cell Counter
- Large-Scale Autoclave
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